VARIANT PROTEIN HAVING DIAPHORASE ACTIVITY

摘要

PROBLEM TO BE SOLVED: To provide a variant diaphorase whose activity level is equal to or higher than a prescribed level. SOLUTION: There provided is a variant diaphorase forming a three-dimensional structure in which a distance between tryptophane at the 60th site from the N-terminal and the first site imino nitrogen of a coenzyme flavin mononucleotide in a three-dimensional structure on the formation of a complex with the coenzyme flavin mononucleotide is larger than the distance in a three-dimensional structure by a wild type diaphorase. A method for screening the variant diaphorase forming a three-dimensional structure in which a distance between tryptophane at the 60th site from the N-terminal and the first site imino nitrogen of the coenzyme flavin mononucleotide in a three-dimensional structure on the formation of complex with the coenzyme flavin mononucleotide by a molecular dynamics simulation is larger than the distance in a three-dimensional structure by a wild type diaphorase. COPYRIGHT: (C)2009,JPO&INPIT