| 摘要 |
It is intended to provide a whole-cell bacterial vaccine composition for preventing whooping cough derived from Bordetella parapertussis containing the whole cell of Bordetella parapertussis, a homogenate of the whole cell or a cell lysate as an immunogen, and a method for producing the same. In order to achieve the above object, the present inventors firstly cultured Bordetella parapertussis under a condition of 37°C for 3 days, adjusted the cell density to 1010 cells/mL, added a formaldehyde solution thereto such that the final concentration of formalin was 0.2%, thereby inactivating the cells. The resulting cells were adjusted to 1010 cells/mL in terms of cell density by adding sterile phosphate-buffered saline, whereby a vaccine composition was obtained. The vaccine composition of the invention was inoculated into a mouse (3.5 weeks of age) and infected with a Bordetella parapertussis suspension. As a result, it was revealed that the mouse has a protective activity against infection with Bordetella parapertussis and further shows the protective activity against infection even at a dose one-thirtieth the common inoculum dose of whooping cough vaccine. |
