摘要 |
<p>Method for the identification and quantification of oncogenic HPV nucleic acids comprising: a) first line screening by means of 5 independent SYBR Green I Real-time PCR assays to determine the total viral load and to identify the presence of one or more of 13 high risk HPV genotypes in the sample; b) second line assays to be applied to samples positives for: - 5 independent TaqMan Real-time PCR assays to determine the presence and the viral load of the most common oncogenic HPV types: HPV types: 16, 18, 31, 45, 33 group (including 33, 52, 58, 67 genotypes). - 6 independent SYBR Green I RT Real-time PCR assays to determine the presence in the sample of the oncogenic transcripts E6/E7 of HPV types 16, 18, 31, 33, 45, 58.</p> |
申请人 |
UNIVERSITA' DEGLI STUDI DI MILANO-BICOCCA;COCUZZA, CLEMENTINA, ELVEZIA, ANNA;BROCCOLO, FRANCESCO |
发明人 |
COCUZZA, CLEMENTINA, ELVEZIA, ANNA;BROCCOLO, FRANCESCO |