发明名称 |
A METHOD OF ENHANCING RECOMBINANT PROTEIN PRODUCTION |
摘要 |
The present invention provides a method of enhanced protein production th at comprises the step of expressing a recombinant gene encoding the protein in eukaryotic cells under conditions in which cleavage of the pro-domain of the protein is inhibited or eliminated. Generally the method of the present invention includes the step of inhibiting or altering the cleavage of a pro- domain of a recombinant protein of interest in order to increase the amount of recombinant protein secreted from a eukaryotic cell. Recombinant proteins that can be prepared using the method of this invention include members of the transforming growth factor-ÿ (TGF-ÿ) superfamily, such as bone morphogen etic proteins. Also provided are genetically engineered cells and polynucleo tides for performing the method of the invention.
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申请公布号 |
CA2688232(A1) |
申请公布日期 |
2008.12.04 |
申请号 |
CA20082688232 |
申请日期 |
2008.05.26 |
申请人 |
INDUCE BIOLOGICS INC. |
发明人 |
PEEL, SEAN A.;TURNER, JEFFREY D.;CLOKIE, CAMERON M. L. |
分类号 |
C12N15/79;C07K14/495;C07K14/51;C12N9/64;C12N15/67;C12N15/85;C12P21/02 |
主分类号 |
C12N15/79 |
代理机构 |
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主权项 |
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地址 |
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