TARGET-ORIENTED WHOLE GENOME AMPLIFICATION OF NUCLIEC ACIDS

摘要

Disclosed herein are methods of amplifying target nucleic acid sequences (e.g., DNA or RNA), particularly from a very small amount of starting material, such as a single cell. These methods involve targeting the amplification of specific sequence(s) by use of sequence- specific primers and random primers for whole genome amplification using multiple displacement amplification. Generally, the provided methods are referred to herein as "target- oriented" whole genome amplification. Starting material for target- oriented whole genome amplification can be any sample containing DNA or RNA, however, the technique is particularly suitable for very small amounts of starting material, such as a few cells, a single cell, or a single nucleus. The methods provide amplified nucleic acid (including the target sequence of interest) that can subsequently be analyzed.