DEVELOPMENT OF SENSITIVE FRET SENSORS AND METHODS OF USING THE SAME
摘要
Intramolecular biosensors are disclosed, including PBP-based biosensors, comprising a ligand binding domain fused to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding ligand. At least one of the donor and fluorescent moieties may be internally fused to the biosensor such that both ends of the internally fused fluorophore are fixed. In addition, methods of improving the sensitivity of terminally fused biosensors are provided. The biosensors of the invention are useful for the detection and quantification of ligands in vivo and in culture.
申请公布号
WO2006044612(A3)
申请公布日期
2008.11.27
申请号
WO2005US36957
申请日期
2005.10.14
申请人
CARNEGIE INSTITUTION OF WASHINGTON;FROMMER, WOLF, B.;OKUMOTO, SAKIKO;LOOGER, LOREN;FEHR, MARCUS
发明人
FROMMER, WOLF, B.;OKUMOTO, SAKIKO;LOOGER, LOREN;FEHR, MARCUS