HAPLOTYPING METHOD BY MULTIPLEX AMPLIFICATION

摘要

The present invention relates to a method for determining the haplotype of a DNA molecule comprising nucleotide variations in at least two separate sites and a kit for conducting such method. The present invention enables to simultaneously identify at least two types of nucleotide variations in at least two separate sites in higher fidelity, contributing to haplotyping of a DNA molecule in a single multiplex amplification. Even though the present invention is carried out in a relatively simple fashion, the results for haplotyping of genes are shown to be much more accurate than any conventional methods.