发明名称 |
Fluorescence Polarization Assays for Acetyltransferase/Deacetylase Activity |
摘要 |
Provided are methods for determining the activity of proteins that modulate the acetylation state of a protein substrate. The methods may be used for determining both acetyltransferase activity and deacetylase activity. The methods involve fluorescence polarization measurements for determining the acetylation state of a substrate peptide. The methods may also be used to identify compounds that modulate the activity of a protein having acetyltransferase or deacetylase activity. Also provided are substrates for acetyltransferase or deacetylase enzymes for use in association with a fluorescence polarization assay.
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申请公布号 |
US2008293081(A1) |
申请公布日期 |
2008.11.27 |
申请号 |
US20060885390 |
申请日期 |
2006.03.03 |
申请人 |
SIRTRIS PHARMACEUTICALS, INC. |
发明人 |
MILNE JILL;CARNEY DAVID |
分类号 |
G01N33/58 |
主分类号 |
G01N33/58 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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