| 摘要 |
This invention describes a method for the separation of unbound labelled probe and probe/target complex in solution, by manipulation of probe and/or probe/target complex. Using these methods, optical detection of the probe/target complex can be performed within the reaction chamber and allows recycling of the unbound probe. The methods are applicable to proteins, nucleic acids and other biological molecules. The method is particularly suited for real-time quantitative PCR.
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