发明名称 |
Pegylated G-CSF polypeptides and methods of producing same |
摘要 |
A method for increasing the stability and uniformity of a PEGylated G-CSF polypeptide having at least one PEG moiety attached to the epsilon amino group of a lysine residue or the N-terminal amino group and at least one PEG moiety attached to a hydroxyl group, comprising subjecting the polypeptide to an elevated pH of above 8.0 for a period of time suitable to remove PEG moieties attached to a hydroxyl group, and reducing the pH to about 8.0 or lower; as well as PEGylated G-CSF polypeptides and compositions produced according to the method and methods for increasing neutrophil levels in a patient using the PEGylated G-CSF polypeptides and compositions. |
申请公布号 |
ZA200709704(B) |
申请公布日期 |
2008.11.26 |
申请号 |
ZA20070009704 |
申请日期 |
2007.11.12 |
申请人 |
MAXYGEN HOLDINGS LTD. |
发明人 |
GERMANSEN, CARSTEN;SONI, BOBBY;RASMUSSEN, GRETHE |
分类号 |
A61K;C07K |
主分类号 |
A61K |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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