发明名称 |
IN VITRO METHOD FOR PROVIDING TEMPLATES FOR DNA SEQUENCING |
摘要 |
The present invention relates to an in vitro method for providing unique templates for DNA sequencing and to a kit, which can be used, when employing the selection method of this invention. The examined DNA is subjected to a DNA transposition reaction and to an amplification reaction. The amplification reaction is carried out in the presence of a fixed primer and a selective primer having a complementary sequence to the end of a transposon DNA. A transposition reaction can be carried out also in the presence of a transposon or transposons, which have different ends enabling the design of selective primers for both ends. Each size of the amplification products represents one template for a sequencing reaction.
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申请公布号 |
CA2295968(C) |
申请公布日期 |
2008.11.25 |
申请号 |
CA19982295968 |
申请日期 |
1998.07.10 |
申请人 |
FINNZYMES OY |
发明人 |
MULTANEN, KIRSI;SAVILAHTI, HARRI;TENKANEN, TUOMAS;SOININEN, TIMO |
分类号 |
C12N15/09;C12Q1/68;C12N15/10;C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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主权项 |
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地址 |
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