| 摘要 |
<p>Vaccine against human cerebrospinal meningitis. Microbial strains isolated during an epidemic are separately cultured in vitro by conventional methods, then passaged (48 hrs) several times (pref. 5-6) in the allantoic cavity of the 7-10 day-old chick embryo. Subcultures of the allantoic fluid are used to inoculate industrial cultures. The product is centrifuged, the microbial pellet resuspended, the organisms inactivated (heat, phenol, CH2O, etc.) and then made up into vaccine doses (5-30 strains) contng. 10 billion dead organisms in 0.1 ml., finally lyophilised. Concd. cultures can be obtd., e.g. 500-100 billion organisms per ml., permitting a single intradermal injection of 0.1 ml. of vaccine.</p> |
