| 摘要 |
The present invention compensates a fluctuation of a light-emitting intensity of a light source. First splitting portions ( 12, 13 ) in the present invention are used to split lights emitted from the light source, and an excitation light with a specific wavelength (lambda<SUB>Ex</SUB>) is incident to a sample element 15. Second splitting portions ( 16, 17 ) are used to split the lights from the sample element portion, and a fluorescence detecting portion 18 is used to measure the fluorescence intensity (I<SUB>Em</SUB>). A reference light detecting portion 19 detects a specific wavelength (lambda<SUB>R</SUB>) of the lights without being selected by the first splitting portion, and then the light intensities detected by the reference light detecting portion are outputted and stored in a spectrum data storage portion 21 . The intensity (I<SUB>Ex</SUB>) of the excitation light is calculated according to the wavelength of the excitation light, the wavelength of the reference light, and the intensity of the reference light in the spectrum data. An output of the fluorescence spectrophotometer is calculated through the measured fluorescence intensity (I<SUB>Em</SUB>) and the calculated intensity (I<SUB>Ex</SUB>) of the excitation light.
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