摘要 |
A method for investigating transport processes in a preferably biological specimen, a laser light beam (2, 3) being guided by means of a scanning apparatus line by line over the specimen within definable specimen regions, and the light proceeding from the specimen being detected by means of a detection apparatus, is characterized, in the interest of the capability of investigating, with high accuracy, processes within the specimen that proceed on a short time scale, in that both an image production light beam (5) for the purpose of observing the specimen and a manipulation light beam (4) for the purpose of manipulating the specimen are used as the laser light beam (2, 3), the image production light beam (5) preceding the manipulation light beam (4) in such a way that pixels of the specimen not yet manipulated by the manipulation light beam (4) are illuminated with the image production light beam (5). |