IMPROVED METHODS, SYSTEMS AND COMPOSITIONS FOR MONITORING ENZYME ACTIVITY AND APPLICATIONS THEREOF

摘要

Methods of monitoring enzyme mediated reactions, and particularly nucleic acid synthesis reactions such as pyrosequencing methods that employ enzymatic reporter systems. The methods and systems provide elevated signal levels as compared to conventional pyrosequencing processes, and/or mediate de-phasing of sequencing analyses employing pyrosequencing or other "sequencing by synthesis" methods.