发明名称 |
MODIFIED MESSENGER RNA STABILIZING SEQUENCES FOR EXPRESSING GENES IN BACTERIAL CELLS |
摘要 |
The present invention relates to methods of producing a polypeptide having biological activity in a bacterial cell, comprising: (a) cultivating a bacterial host cell in a medium conducive for production of the polypeptide, wherein the bacterial host cell comprises a nucleic acid construct comprising a promoter region operably linked to a polynucleotide sequence encoding the polypeptide and a modified mRNA processing/stabilizing sequence located downstream of the promoter region and upstream of the ribosome binding site of the polynucleotide sequence encoding the polypeptide, wherein the modified mRNA processing/stabilizing sequence promotes higher expression of the polynucleotide sequence compared to an unmodified mRNA processing/stabilizing sequence; and (b) isolating the polypeptide having biological activity from the cultivation medium. The present invention also relates to such modified mRNA processing/stabilizing sequences, nucleic acid constructs, and bacterial host cells and to methods of obtaining such bacterial host cells. |
申请公布号 |
WO2008140615(A2) |
申请公布日期 |
2008.11.20 |
申请号 |
WO2007US88060 |
申请日期 |
2007.12.19 |
申请人 |
NOVOZYMES, INC.;THOMAS, MICHAEL;ERICHSEN, GLORIA;WIDNER, WILLIAM |
发明人 |
THOMAS, MICHAEL;ERICHSEN, GLORIA;WIDNER, WILLIAM |
分类号 |
C12N1/00 |
主分类号 |
C12N1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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