METHOD FOR RAPID DETECTION OF CLOSTRIDIUM BOTULINUM

摘要

The invention provides a rapid sensitive, specific and robust universal assay for detecting all C. botulinum toxin types in a sample by (a) PCR amplifying DNA in the sample by use of particular primers, (b) detecting the presence or absence of amplified DNA, and (c) correlating the presence or absence of amplified DNA with the presence or absence, respectively, of C. botulinum in the sample. The invention further provides a method for type-specific analysis of C. botulinum toxin gene types A-G in a sample utilizing particular primers and optional probes that are specific to each C. botulinum toxin type.