| 摘要 |
The invention relates to methods for making a peptide standard for mass s pectrometry said method comprising (a) identifying endopeptidase cleavage si tes in a parent polypeptide sequence of interest; (b) selecting peptide sequ ences from said parent polypeptide which are defined by endopeptidase cleava ge sites of step (a); (c) adding a C-terminal extension to each selected seq uence; wherein if the endopeptidase cleavage site is C-terminal to its recog nition sequence then the C- terminal extension comprises 1 to 6 amino acids, wherein if the endopeptidase cleavage site is N-terminal to its recognition sequence then the C-terminal extension comprises said recognition sequence, wherein if the endopeptidase cleavage site is within its recognition sequen ce then the C-terminal extension comprises the remainder of said recognition sequence C-terminal to the cleavage site; and (d) synthesising a peptide ha ving the extended amino acid sequence of step (c). The invention also relate s to peptide standards and methods of analysing samples.
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