CELLOBIOHYDROLASE-ENCODING NUCLEOTIDE SEQUENCES WITH REFINED TRANSLATIONAL KINETICS AND METHODS OF MAKING SAME

摘要

Provided are polynucleotide sequences and synthetic genes encoding cellobiohydrolase enzymes for expression in a host organism with improved and/or refined translational kinetics, and methods of making same. The resultant cellobiohydrolase-encoding nucleotide is predicted to be translated rapidly along its entire length. Expression of the resultant cellobiohydrolase-encoding nucleotide is predicted to result in improved protein expression levels in cases where inappropriate or excessive translation pauses reduce protein expression. In addition, expression of the resultant cellobiohydrolase-encoding nucleotide is predicted to result in improved levels of active and/or natively folded and functional polypeptide expression in cases where inappropriate or excessive translational pauses causes expression of inactive, insoluble, aggregated or somehow dysfunctional or minimally active cellobiohydrolase.