KIT AND METHOD FOR THE DETECTION AND IDENTIFICATION OF CLINICALLY RELEVANT YEASTS, USING AN ISOTHERMAL DNA AMPLIFICATION FOLLOWED BY THE HYBRIDISATION TO SPECIES- SPECIFIC OLIGONUCLEOTIDE PROBES, AND RESPECTIVE APPLICATIONS

摘要

The present invention is related with a method for the detection and identification of clinically relevant yeasts using an isothermal technique for DNA amplification with non-specific ribosomal DNA-targeted primers (e.g. rDNA 26S), followed by reverse hybridisation of the labelled products (e.g. with digoxigenin) against a panel of species-specific oligonucleotide probes (e.g. immobilized onto a solid matrix as a nylon membrane). Hybridisation occurrence is detected by a colorimetric method. The present invention is also related with the development of a diagnostic kit for the detection and identification of clinically relevant yeasts, based in the referred method. The present invention is useful for the detection and identification of yeasts related with infections and has the advantages of being a simple, fast, reliable and affordable method with applications in the clinical, pharmaceutical, veterinary, food and biotechnology areas.