发明名称 |
METHOD FOR MICROPROPAGATION OF MONOCOTS BASED ON SUSTAINED TOTIPOTENT CELL CULTURES |
摘要 |
<p>The present invention provides a method of micropropagating a monocotyledonous plant comprising: (a) cultivating an explant of tissue from a monocotyledonous plant shoot tip on a primary medium, wherein the explant has been pretreated with a cold temperature and the primary medium comprises auxin or auxin and cytokinin, to produce a totipotent embryogenic cell culture; (b) treating the totipotent embryonic cell culture with a cold temperature; (c) maintaining the totipotent embryogenic cell culture by cultivation on a secondary medium, whereby a totipotent embryogenic cell culture of a monocotyledonous plant is produced and maintained; and (d) transferring the embryogenic cell culture of step (c) to a tertiary medium to continue multiplication and to produce a plantlet with roots and shoots, thereby micropropagating a monocotyledonous plant. The micropropagation techniques described herein provide plants for such purposes as development of elite plant lines, phytoremediation and biomass production.</p> |
申请公布号 |
WO2008136797(A1) |
申请公布日期 |
2008.11.13 |
申请号 |
WO2007US11008 |
申请日期 |
2007.05.07 |
申请人 |
UNIVERSITY OF SOUTH CAROLINA;MARTON, LAZLO;CZAKO, MIHALY |
发明人 |
MARTON, LAZLO;CZAKO, MIHALY |
分类号 |
A01H4/00;A01H17/00;C12N15/82 |
主分类号 |
A01H4/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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