An automatic bacteria sampling apparatus can minimize the analysis error by sterilizing and washing the pipeline in a standby mode. An automatic bacteria sampling apparatus(100) comprises a case(102) having a receiving portion(104), an introduction pipe(106), a discharge line(108), and a heater(110). The introduction pipe is arranged in the case and connected to one side of the receiving portion. The discharge line is arranged in the case and is connected to the other side of the receiving portion. The heater is installed adjacent to the introduction pipe.