RECOMBINANT PLASMID DNA, ENCODING HUMAN FACTOR VII PROTEIN SEQUENCE, CELL LINE BHK/F7, TRANSFORMED WITH PLASMID DNA

摘要

FIELD: chemistry, biotechnology. ^ SUBSTANCE: invention relates to field of biotechnology and concerns obtaining factor VII protein by method of recombinant DNA. Recombinant plasmid DNA was constructed for expression of blood clotting factor VII in mammalian cells, which is product of ligating of fragment of cDNA of human factor VII gene, flanked by sites of restrictases Xhol and BamHI recognising, with large XhoI/BglII fragment of vector pEFZeo, including genes of resistance to ampicillin and zeocin. As result of BHK cell transformation with new recombinant plasmid, cell line BHK/F7 was obtained, which produces recombinant protein of factor VII with output of up to 40 mkg/ml. ^ EFFECT: obtaining cell line producing recombinant protein of factor VII. ^ 2 cl, 4 dwg, 4 ex