MICRO RNA ARRAYS AND METHODS OF USING THE SAME

摘要

Isolated polynucleic acids are disclosed. In some embodiments, the isolated polynucleic acids are selected from the group consisting of (a) a polynucleic acid comprising a nucleic acid sequence set forth in Table 1 or Table 2; (b) a polynucleic acid comprising a nucleic acid sequence having at least about 90% identity to a nucleic acid sequence set forth in Table 1 or Table 2; and (c) a polynucleic acid capable of hybridizing under stringent conditions to a nucleic acid sequence set forth in Table 3 or Table 4, wherein the polynucleic acid is 50 nucleotides or shorter. Also disclosed are libraries of isolated polynucleic acids; kits for determining the presence of miRNA expressed in a sample; methods for detecting, quantifying, or both at least one miRNA directed against at least one specific gene present in a sample; methods for designing a polynucleotide primer sequence for amplifying a precursor miRNA (pre-miRNA), a mature miRNA, or both of interest; methods for detecting at least one sequence variation in at least one miRNA in a sample; computer program products including computer-executable instructions embodied in a computer-readable medium for performing the disclosed methods; and computer systems that in some embodiments include relational databases.