DETECTION OF BLOOD PLASMA SCHIZADRIN B OF DISSIPATING BLOOD STASIS BOTANICAL

摘要

A detection method of blood plasma schizadrin B of dissipating blood stas is botanical is disclosed. The method includes: (1) extracting schizadrin B from plasma of mammalian administered dissipating blood stasis botanical by ethyl acetate with the volume ratio 1:4, whirling 3-5mins, centrifugating at 9600rpm for 10mins, drying and enriching the upper layer at 25-30°C, and re dissolving with mobile phase; (2) UPLC/MS measuring: UPLC condition: chromat ographic column: Acquity UPLC BEH C18, 2.1´100mm, mobile phase A: water-acet onitrile-formic acid 95:5:0.1 v/v/v, mobile phase B: acetonitrile-formic aci d 100:0.1 v/v; MS condition: electric spraying ion source(ESI), detecting wi th positive ion mode, scanning at the range of m/z 150-1200. The method can be used for pharmacokinetics study of schizadrin B in dissipating blood stas is botanical.