The invention relates to a device for reading the fluorescence emitted by chromophorous members associated with biological or chemical components at the surface of an object (10), that comprises means (12) for lighting the chromophorous members with an excitation light supplied by a laser source (16) and flowing into a wave guide formed by a spoke (18) of a transparent material, and means (14) for collecting and picking up the fluorescence from the chromophorous members, said means (14) including a large field optical system (34).
