RECOMBINANT VITAMIN K DEPENDENT PROTEINS WITH HIGH SIALIC ACID CONTENT AND METHODS OF PREPARING SAME

摘要

Methods of isolating highly sialylated recombinant vitamin K dependent pr oteins, particularly Factor IX, by chromatographic methods are described. Th e highly sialylated recombinant proteins are characterized. The improved Fac tor IX has at least 62% N- glycosylation with 3 or 4 sialic acid residues an d improved bioavailability and pharmokinetic properties.