Culture Double Container and a Method of Culture

摘要

A culture container and a method of culture which do not require a plurality of incubators in a case where a plurality of cells of different kinds which like different gas concentrations should be cultured simultaneously. A culture double container 1 comprises a gas-permeable culture container 2 having an inlet and an outlet 6 , a gas-impermeable container 3 covering the culture container 2 , and joint portions 12 and 13 for holding the culture container 2 in the gas-impermeable container 3 in such a manner that space is defined between the culture container 2 and the gas-impermeable container 3 . The culture double container 1 receives gas controlled in its composition in the space defined houses the culture container 2 and the gas-impermeable container 3 during culture of cell or micro-organism.