SEQUENTIAL ANALYSIS OF BIOLOGICAL SAMPLES

摘要

Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing m ultiple targets adhered to a solid support, binding at least one fluorescent probe to one or more target present in the sample, and observing a signal f rom the fluorescent probe. The method further includes the steps of oxidizin g the bound fluorescent probe with a solution containing an oxidizing agent that substantially inactivates the fluorescent probe, binding at least one f luorescent probe to one or more target present in the sample, and observing a signal from the fluorescent probe. The methods disclosed herein also provi de for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.