DETECTION OF BLOOD PLASMA AMYGDALIN OF DISSIPATING BLOOD STASIS BOTANICAL

摘要

A detection method of blood plasma amygdalin of dissipating blood stasis botanical is disclosed. The method includes: (1) adding 2-5mol/L phosphoric acid to plasma of mammalian administered dissipating blood stasis botanical and mixing the solution of plasma-phosphoric acid (1:2-3, v/v), applying the solution to small column of Waters Oasis HLB activated by methanol and wate r; after leaching by water and 80-100% methanol and eluting by 0.2-1% ammoni a-methanol, drying and enriching the eluent at 25-30°C; and redissolving wit h mobile phase; (2) UPLC/MS measuring: UPLC condition: chromatographic colum n: Acquity UPLC BEH C18, 2.1´100mm, mobile phase A: water-acetonitrile-formi c acid 95:5:0.1 v/v/v, mobile phase B: acetonitrile-formic acid 100:0.1 v/v; MS condition: electric spraying ion source(ESI), detecting with positive io n mode, scanning at the range of m/z 150-800. The method can be used for pha rmacokinetics study of amygdalin of dissipating blood stasis botanical.