发明名称 |
METHOD OF SITE-SPECIFICALLY INTRODUCING NON-NATURAL AMINO ACID INTO PROTEIN USING MITOCHONDRIAL PROTEIN AND METHOD FOR EFFECTIVELY PREPARING tRNA |
摘要 |
This invention is intended to provide a protein synthesis system used for producing a tryptophan analogue-containing non-natural-amino-acid-containing protein that satisfies the following conditions: (i) tRNA that transfers a non-natural amino acid is not recognized by an endogenous aminoacyl tRNA synthetase (aaRS); (ii) it is recognized selectively by aaRS exclusive for a non-natural amino acid; and (iii) endogenous tRNA is not recognized by aaRS exclusive for a non-natural amino acid. In the eukaryotic organism-derived cell-free protein synthesis system, a yeast mitochondrial tryptophanyl tRNA synthetase is used in combination with mitochondrial tRNA Trp (mt tRNA Trp ). |
申请公布号 |
EP1818400(A4) |
申请公布日期 |
2008.11.05 |
申请号 |
EP20050811383 |
申请日期 |
2005.11.22 |
申请人 |
GIFU UNIVERSITY |
发明人 |
NISHIKAWA,KAZUYA;YOKOGAWA, TAKASHI;OHNO, SATOSHI |
分类号 |
C12N15/09;C07K16/40;C12N9/00;C12P19/34;C12P21/02;C12Q1/25 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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