发明名称 |
PROCEDE DE PRODUCTION DE L'ACIDE 7-AMINO DESACETOXY CEPHALOSPORANIQUE |
摘要 |
1324159 Producing 7-amino-desacetoxycephalosporanic acid TOYO JOZO CO Ltd 14 March 1972 [31 May 1971] 11915/72 Heading C2A [Also in Division C3] 7 - Amino - desacetoxycephalosporanic acid having the formula is produced by N-deacylating a water-soluble salt of 7-phenylacetamido- or 7-phenoxyacetamido-desacetoxycephalosporanic acid with a deacylating enzyme produced by Bacillus megaterium B-400, FERM-P No. 748. The enzyme may be in the form of a culture filtrate of the micro-organism or a purified preparation derived from the filtrate, e.g. the enzyme adsorbed on a non-inactivating carrier (see Division C3). The preferred water-soluble salt of the cephalosporin is the Na or K salt, and the N-deacylation of the salt is effected either by incubating it insolution in a culture filtrate of the micro-organism or by passing its solution through a column of the enzyme adsorbed on an insoluble inert carrier, at pH 7-8 and 30-45‹ C. using a concentration of the salt of 0À1 to 20 mgs./ml. The 7-aminodesacetoxycephalosporin may be recovered by bringing the solution to its iso-electric point (pH 3.7), concentrating, cooling to precipitate the product and washing the precipitate with acetone and drying. |
申请公布号 |
BE780676(A1) |
申请公布日期 |
1972.09.15 |
申请号 |
BE19720780676 |
申请日期 |
1972.03.15 |
申请人 |
TOYO JOZO CY LTD, 632-1, OHOHITO-CHO MIFUKU, TAGATA-GUN, SHIZUOKA (JAPON), |
发明人 |
T. WATANABE;T. YAMAGUCHI;K. MATSUMOTO;T. FUJII |
分类号 |
A61K31/545;C07D501/18;C12N9/80;C12P35/02;C12R1/11 |
主分类号 |
A61K31/545 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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