| 摘要 |
A method of collecting a desired lower density component of buffy coat or a higher density component of buffy coat comprising: providing a separator having an inlet, a first outlet, and a second outlet; adding an anticoagulant fluid to whole blood obtained from a patient or a blood donor in a predetermined ratio to form a mixture of whole blood and anticoagulant fluid; pumping the mixture of whole blood and anticoagulant fluid into the separator via the inlet at a selected inlet rate; separating the mixture into blood components of different densities; withdrawing plasma and red blood cells from the separator while continuing to pump the mixture of whole blood and anticoagulant fluid into the separator, the plasma and red blood cells being withdrawn at rates so as to build up buffy coat in the separator, the plasma being withdrawn via the first outlet and the red blood cells being withdrawn via the second outlet; and upon a predetermined amount of buffy coat building up in the separator, collecting the buffy coat from the separator by discontinuing the withdrawal of red blood cells from the second outlet, thereby causing the red blood cells to push the buffy coat out of the separator via the first outlet; rinsing the separator with a saline solution; pumping the collected buffy coat back to the separator via the inlet; and separating the collected buffy coat into a higher density buffy coat layer and a lower density buffy coat layer. |
