| 摘要 |
<p>THE METHODS AND APPARATUS [100] DISCLOSED HEREIN CONCERN DNA SEQUENCING. IN SOME EMBODIMENTS OF THE INVENTION, THE METHODS COMPRISE MEASURING THE DISTANCE BETWEEN LABELED NUCLEOTIDES [220], SUCH AS NUCLEOTIDES LABELED WITH BULKY GROUPS. THE METHODS MAY FURTHER COMPRISE PLACING IDENTICAL TEMPLATE DNA [200] INTO FOUR REACTION CHAMBERS [110,120,130,140], EACH CONTAINING A DIFFERENT LABELED NUCLEOTIDE PRECURSOR, SYNTHESIZING COMPLEMENTARY STRANDS [230, 240, 250] AND DETECTING LABELED NUCLEOTIDES [220]. THE DISTANCES BETWEEN LABELED NUCLEOTIDES [220] MAY BE USED TO CONSTRUCT [450] DISTANCE MAPS [310,320,330,340] FOR EACH TYPE OF LABELED NUCLEOTIDE [220]. THE DISTANCE MAPS [310,320, 330,340] MAY BE ALIGNED [520] TO OBTAIN A NUCLEIC ACID SEQUENCE [210]. OVERLAPPING DATA ANALYSIS AND FREQUENCY ANALYSIS MAY BE USED TO CONSTRUCT [450] THE DISTANCE MAPS [310,320,330,340] AND NON-OVERLAPPING DATA ANALYSIS MAY BE USED TO ALIGN [520] THE DISTANCE MAPS INTO A SEQUENCE [210].</p> |
