TENOCYTE CONTAINING BIOSCAFFOLDS AND TREATMENT USING THE SAME

摘要

The present invention relates to methods for preparing bioscaffolds usefu l in the repair of tears. More specifically, the invention relates to a meth od of treating rotator cuff tear in a mammalian subject in need thereof comp rising the steps of: (i) selectively expanding tenocytes in vitro in culture medium comprising insulin or a functional derivative and a glucocorticoid o r a glucocorticoid-like molecule to produce a culture of expanded tenocytes; (ii) seeding a bioscaffold with said expanded tenocytes to produce a tenocy te seeded bioscaffold; and (iii) implanting said tenocyte seeded bioscaffold proximal to the rotator cuff tear. The present invention also relates to a bioscaffold comprising cells, wherein more than 80 % of said cells are tenoc ytes.