Method of Converting Water-Soluble Active Proteins Into Hydrophobic Active Proteins, the Use of the Same for the Preparation of Monomolecular Layers of Oriented Active Proteins, and Devices Comprising the Same

摘要

The present invention relates to a method of converting hydrophilic active proteins (HPiAP) into hydrophobic active proteins (HPoAP) suitable for the anchorage in their active form on hydrophobic substrates. The present invention also relates to the preparation of ordered monomolecular layers of oriented active proteins immobilized onto hydrophobic solid supports to be used for mechanical manipulation and investigations, including Atomic Force Microscopy (AFM) in aqueous solutions and assays employing the same devices.