ADENOVIRAL VECTOR COMPRISING HERPES SIMPLEX VIRUS TYPE 1 THYMIDINE KINASE AND A TRANSGENE FOR INCREASING THE EXPRESSION OF THE TRANSGENE

摘要

Compositions and methods useful in transgene expression are provided. Herpes simplex virus type 1 thymidine kinase sequences ("TK sequences") are used to enhance transgene expression in first generation and high capacity adenoviral vectors. An mCMV promoter-driven ß-galactosidase-expressing cassette is combined with TK sequences through direct fusion of the cDNA's. ß-galactosidase (transgene) expression is enhanced independent of adenoviral vector selection. Methods of enhancing transgene expression employing the inventive adenoviral vectors are provided, along with pharmaceutical preparations comprising the inventive vectors and kits for enhanced transgene expression.