发明名称 Procedure for the treatment of vital plant, animal cells and/or cell clusters with pulsed electric energy for the disruption of the cells, comprises perforating cell membranes in irreversible and reversible manner through poration reactor
摘要 The procedure for the treatment of vital plant, animal cells and/or cell clusters with pulsed electric energy for the disruption of the cells and/or cell membrane, comprises perforating cell membranes in pumpable and agglomerate product in reversible and irreversible manner through a poration reactor by adjusting a high current density and optimal surface charge density. The current density (J) of 50-500 kA/m2> is reached through initiating a unipolar or bipolar current pulse in sufficient pulse voltage. The surface charge density in the reaction zone of each energy impulse is 0.5-2 C/m2>. The procedure for the treatment of vital plant, animal cells and/or cell clusters with pulsed electric energy for the disruption of the cells and/or cell membrane, comprises perforating cell membranes in pumpable and agglomerate product in reversible and irreversible manner through a poration reactor by adjusting a high current density and optimal surface charge density. The current density (J) of 50-500 kA/m2> is reached through initiating a unipolar or bipolar current pulse in sufficient pulse voltage. The surface charge density in the reaction zone of each energy impulse is 0.5-2 C/m2>. The product to be treated, pumpable and agglomeratable is directed at stationary electrode system and is immersed in a fluid and/or the cell consists of cell fluid, returned activated cell fluid or aqueous fluid emerged through crushing. The conductivity of the fluid is 0.5-5 mS/cm and is adjusted to 0.1-20 mS/cm. Pumpable meat and fish product, plant product such as fruit and vegetable mash and/or agglomerated product are treated. A system pressure in the reaction zone is adjusted by over pressure of 0-10 bar in the mash with gas inclusion. The over pressure in the agglomerated product is 0-0.5 bar. A limited pulse quantity of 20-60 pulse is delivered to each volume element and between the pulses, a period of 20-40 milliseconds is passed. A continuous product throughput is reached to 5,000-15,000 kg/h and a continuous treatment of pumpable and agglomerated product is realized. An independent claim is included for a poration reactor for pumpable and agglomerate product.
申请公布号 DE102007016927(A1) 申请公布日期 2008.10.16
申请号 DE20071016927 申请日期 2007.04.05
申请人 KERN, MARTIN 发明人
分类号 B01J19/08;C12N13/00 主分类号 B01J19/08
代理机构 代理人
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