| 摘要 |
The invention relates to a composition for inhibiting the expression of a target gene in a eukaryotic cell by RNA interference, the composition compr ising a genetic construct and the target gene being introduced into the euka ryotic cell by means of said construct, and the construct having a first tar get gene and a first siRNA tag. The invention also relates to a composition for inhibiting the expression of one or more target genes in a eukaryotic ce ll by RNA interference. The composition comprises at least two genetic const ructs and the target genes are introduced into the eukaryotic cell by means of said constructs, a) the first construct containing a first target gene an d a first siRNA tag and b) the second construct containing a second target g ene and a second siRNA tag, and the first and the second siRNA tag having di fferent nucleic acid sequences. The invention finally relates to a method fo r inhibiting the expression of a target gene in a eukaryotic cell. Said meth od comprises the following steps: a) providing at least one eukaryotic cell, said cell being capable of RNA interference, b) transfecting the cells with a composition according to the invention and c) introducing at least one si RNA that is complementary to a siRNA tag of a transfected construct to inhib it the expression of the target gene that formsa transcription unit together with the siRNA tag.
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