OPTIMIZATION OF CORONATINE PRODUCTION IN A GENETICALLY IMPROVED STRAIN OF PSEUDOMONAS SYRINGAE
摘要
Stable genetically engineered bacterial strains that overproduce coronatine are provided. The stable strains can be successfully cultivated to overproduce coronatine at temperatures that are suitable for large scale, commercial preparations of coronatine. The overproducing strains are also non-pathogenic. An exemplary strain is Pseudomonas syringae APVl, which successfully overproduces coronatine at 26°C. Methods of optimizing culture conditions for coronatine production from the novel stable overproducing strains are provided, as are methods for using the overproducing strains to induce abscission and increase taxane production.
申请公布号
WO2007127835(A3)
申请公布日期
2008.10.16
申请号
WO2007US67487
申请日期
2007.04.26
申请人
THE BOARD OF REGENTS FOR OKLAHOMA STATE UNIVERSITY;BENDER, CAROL, L.;PENALOZA-VAZQUEZ, ALEJANDRO