| 摘要 |
Methods of evaluating the composition of a cell culture (e.g., to disting uish chondrocytes from fibroblasts) and methods for evaluating the phenotype of an individual cell (e.g., as a chondrocyte) are disclosed. The methods m ay be used, for example, for assessing chondrocyte cultures used for treatme nt of cartilage defects. In some embodiments, the invention involves identif ying cell culture composition or the identity of a cell based on expression level of a fibroblast marker. In other embodiments, the invention involves c omparing expression levels of at least one chondrocyte marker and at least o ne fibroblast marker in a cell culture sample or in an individual cell. In i llustrative embodiments, the chondrocyte marker is hyaluronan and proteoglyc an link protein 1 (HAPLN1 ), and the fibroblast marker is microfibrillar ass ociated protein 5 (MFAP5).
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