METHODS FOR PURIFYING ADENO-ASSOCIATED VIRUS VIRIONS

摘要

The present invention provides methods for purifying adeno-associated virus (AAV) virions, including recombinant AAV virions, generally involving binding of an AAV virion comprising a variant capsid protein comprising a purification peptide to a binding moiety that binds the purification peptide. The present invention further provides nucleic acids comprising nucleotide sequences encoding the variant capsid proteins; as well as recombinant AAV virions comprising the variant capsid proteins.