NOVEL 2,3-SIALYLTRANSFERASE AND METHOD FOR PRODUCING COMPOUND HAVING GALATOSE IN TERMINAL USING THE SAME

摘要

A novel 2,3-sialyltransferase is provided to mass-produce inexpensively and simply a substance having galatose in its terminal such as 2,3-sialyl lactose which is contained in human milk and inhibits bacterial and viral infection by having strong affinity with influenza A virus hemagglutinin(HA). A novel 2,3-sialyltransferase having the amino acid sequence of SEQ ID NO:1 is isolated from Haemophilus ducreyi(KCTC 2745). A 2,3-sialyltransferase mutant is produced by substituting an amino acid, tyrosine(Tyr) or praline(Pro) at 21 or 112 amino acid position with another amino acid, cysteien(Cys) or glutamine(Gln), respectively. A substance having galatose in its terminal, 2,3-sialyl lactose is produced by reacting a mixture containing CMP(cytidine monophosphate)-N-acetyl neuraminic acid and lactose with 2,3-sialyltransferase or its mutant.