| 摘要 |
<p>A process for the detection of antibodies in a test sample by carrying out the following steps: (a) preparing an essentially isotonic and low ionic strength suspension comprising said test sample and erythrocytes; (b) incubating the erythrocytes, test sample and low ionic strength medium at 37-45°C for various time periods to optimize antibody uptake; (c) combining said suspension with an amount of a solution of hexadimethrine bromide which is effective for agglutination of said erythrocytes and (d) separating the resultant agglutinates of polymer and erythrocytes from said suspension; (e) neutralizing the effect of hexadimethrine bromide by adding an effective amount of a gylcosaminoglycan in combination with other polybrene neutralizing agents; (f) monitoring the resuspended agglutinates for the presence or absence of antibody, (g) stabilizing the antibody dependent aggregate by adding an effective macromolecule as a component of the dispersing solution or separately and optionally (h) packing and reincubating the test red cells to further increase the test sensitivity.</p> |
