发明名称 |
Exhaustive selection of RNA aptamers against complex targets |
摘要 |
A method of identifying RNA ligands which bind to a target molecule by treating a first pool of RNA ligands that collectively bind more than one target under conditions effective to reduce the concentration or eliminate the presence of one or more predominate target-binding RNA ligands from the first pool of RNA ligands; amplifying the RNA ligands in the treated first pool, thereby forming a second pool of RNA ligands that is enriched in one or more non-predominate target-binding RNA ligands of the first pool but not the one or more predominate target-binding RNA ligands thereof; and identifying one or more predominate target-binding RNA ligands that are present in the second pool at a higher concentration than other target-binding RNA ligands. Oligonucleotides and kits which can be used in practicing the present invention are also disclosed, as are aptamers that bind to a heat shock factor protein and their use.
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申请公布号 |
US7435542(B2) |
申请公布日期 |
2008.10.14 |
申请号 |
US20030602837 |
申请日期 |
2003.06.24 |
申请人 |
CORNELL RESEARCH FOUNDATION, INC. |
发明人 |
SHI HUA;LIS JOHN T. |
分类号 |
C12Q1/68;C12N15/10;C12N15/11;C12N15/115;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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