THROMBIN DERIVATIVE AND METHOD FOR PREPARATION THEREFORE, ANHYDROTHROMBIN DERIVATIVE AND METHOD FOR PREPARATION THEREFORE, COMPOSITION FOR CAUSING PLATELET AGGREGATION, METHOD FOR CAUSING PLATELET AGGREGATION, CLINICAL TEST DRUG, METHOD FOR CLINICAL TEST, INHIBITOR FOR THROMBUS FORMATION, ADSORBENT AND METHOD FOR PREPARATION THEREFORE, METHOD FOR PREPARATION OF PURIFIED BLOOD COAGULATION FACTOR ?

摘要

A thrombin derivative having a lowered fibrinogen activity, a process for producing thereof, an anhydrothrombin derivative having a lowered fibrinogen activity, and a process for producing thereof are provided. This invention is to provide a thrombin derivative formed by modifying carboxyl group(s) of the thrombin and an anhydrothrombin derivative formed by modifying carboxyl group (s) of the anhydrothrombin. The thrombin derivative can be utilized as a composition for inducing platelet aggregation and further as a reagent of specific aggregation for clinical testing and the like. By the affinity chromatography having carboxyl group(s) bound with anhydrothrombin as a ligand, it is made possible to carry out necessary purification with high selectivity relative to the blood coagulation factor VIII. Further, the anhydrothrombin derivative which results from modifying carboxyl group(s) of anhydrothrombin produces sufficient antithrombogenic effects at a smaller amount as compared with the previous anhydrothrombin and, therefore, can be utilized as an agent for inhibiting the thrombogenesis.