| 摘要 |
FIELD: medicine. ^ SUBSTANCE: single blood sampling of funic blood from one donor carry out in two stages in continuous regimen, at the first stage spend a blood sampling by exfusion from distal department of an umbilical vein with the subsequent hashing with anticoagulant in the container 1 during all procedure of the collecting; at the second stage, after placenta birth, spend blood sampling which have remained in her by perfusion. To do that place the placenta in tray root part upwards, carry out a puncture of proximal department of an umbilical vein adjoining to the placenta, force in it anticoagulant from the container 2 to swelling, lift the placenta so that blood being in it together with anticoagulant followed in the container, after fall of vein placenta palpate, taking the blood rests, then freshly plucked exfusate and perfusate unite by transfer of blood from the container 2 in the container 1, add peer volume of Polyglucinum, an admixture mix, maintain within 50-60 minutes, separate supernatant fluid, centrifugate it at 2000 rpm, remove plasma, to the received deposit containing a blast concentrate, add equal volume of 10-12% of the dimethylsulfoxide solution in Polyglucinum, before carrying out of the subsequent freezing an admixture place in cryobag, Impose on it thermocouple with a range of measured temperatures from -200°C to +500°C, connected to a multichannel measuring instrument-regulator of temperatures of IRT-4/16 with which help throughout all period of cooling make temperature measurement, carry out giving on the computer of the information in the form of a graphic curve, make conservation of the set parametres in non-volatile memory, then to the received sample containing a target product, intended for storage in the frozen kind, apply the received graphic curve of depression of temperature and the passport containing all qualitative for subsequent transplantation qualitative and quantitative characteristics, including quantity teleorganic blasts. Method allows to collect from one donor at the single collecting a maximum quantity of funic blood and to allocate the greatest possible quantity of the blasts, necessary for carrying out of successful transplantation. ^ EFFECT: guarantee and reliability of maximum conservation of blasts viability increases. |
