发明名称 |
REPORTER CONSTRUCT FOR DETECTING NOTCH SIGNAL TRANSMISSION IN HIGH SENSITIVITY |
摘要 |
<P>PROBLEM TO BE SOLVED: To provide a screening system that has high detection accuracy and performs accurate screening and a screening system that measures an endogenous Notch signal transmission system. <P>SOLUTION: The nucleic acid molecule comprises at least three RBP-Jκ binding sequences in which the RBP-Jκ binding sequence is TGGGAA. The method for screening a substance for adjusting a Notch signal transmission system comprises (a) a process for bringing cells that contain the nucleic acid molecule and a reporter protein encoding sequence operably connected to a promoter sequence and expresses an endogenous Notch protein or a constructionally active Notch protein into contact with a candidate substance and (b) a process for assaying the expression amount of the reporter protein in the cells. <P>COPYRIGHT: (C)2009,JPO&INPIT |
申请公布号 |
JP2008237068(A) |
申请公布日期 |
2008.10.09 |
申请号 |
JP20070080269 |
申请日期 |
2007.03.26 |
申请人 |
OSAKA UNIV;SHIONOGI & CO LTD |
发明人 |
OKOCHI MASAYASU;YANAGIDA KANTA;TAKEDA MASATOSHI |
分类号 |
C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12Q1/02;G01N33/15;G01N33/50 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|