GENETIC SEX DETERMINATION METHOD FOR FLOUNDER AND PRIMER TO BE USED IN GENETIC SEX DETERMINATION METHOD

摘要

PROBLEM TO BE SOLVED: To surely determine the genetic sex of a flounder before the sex differentiation period. SOLUTION: DNA of a flounder is subjected to PCR process by using a primer comprising an oligonucleotide composed of the base of 5'-GTGGACATTT GTACTCCACA GACCA-3' and 5'-GTGAGCGGGT ACATGTGTGT GAG-3' concerning Poli185TUF or 5'-CCTCACAAAG ATATTTGTAC AGGTGCA-3' and 5'-CATCTTTAGG TCACATTGTC ACTGCTG-3' concerning Poli109TUF, the obtained PCR product is subjected to gel electrophoresis and the flounder is judged to be male when the electrophoretic product has a band of 212 bp for Poli185TUF or the product has a band of 144 bp for Poli109TUF. COPYRIGHT: (C)2009,JPO&INPIT