Biosensors with Improved Sensitivity

摘要

A sensor is described for use with at least one optical detector ( 5 ), the sensor comprising a substrate ( 11 ) with optical outlets, a porous membrane ( 12 ) and micro fluidic channels ( 13 ) for conducting analyte fluid towards sensing locations ( 10 ) of the porous membrane ( 12 ). The sensing locations ( 10 ) are adapted for at least restraining light variable molecules ( 23 ) which bind to analytes to be determined. The optical output of the light variable molecules changes when they are in close proximity to a target molecule. The micro fluidic channels ( 13 ) are shaped to reflect light emitted from the sensing locations ( 10 ) towards the optical outlets and the substrate has diffracting optical elements ( 15 ) aligned with microfluidic channels to diffract light towards the optical outlets. The diffracting optical elements can be lenses.